侯尚国课题组主要从事多维生物动态成像方向研究。研究方向包括：

1. 多维动态光学显微成像技术开发；

2. 病原体与宿主相互作用机制；

3. 定量生物分子动态成像；

4. 人工智能生物成像。

侯尚国博士的研究领域为高时空分辨光学显微成像技术开发及其在生物体系中的应用。针对不同尺度的分子和细胞体系，发展了一系列的多光学维成像方法，用于研究各种不同体系和条件下的生物分子与细胞之间的相互作用。研究成果以通讯以及第一作者身份发表在Nature Communications (×3)、Advanced Science、Science Bulletin等国际高水平期刊。研究工作被Nature Photonics、Molecular Cell、ACS Nano等权威杂志正面引用，并多次被Phy.org、Duke Research 等学术网站亮点报道。主持5项国家自然科学基金项目以及省市等科研基金项目。指导课题组学生获得深圳湾实验室主任特等奖学金（2023）、中国研究生电子设计竞赛华中赛区二等奖（2023）。

2022：广东省珠江人才计划-青年拔尖人才

2021：深圳市高层次人才

2020：杜克大学Fitzpatrick光子学研究所杰出博后讲座奖

2019：杜克大学杰出博后提名奖

2015：上海市优秀毕业生

1. Hao Sha†, Yu Wu†, Yongbing Zhang†*, Xiaochen Feng, Haoyang Li, Zhong Wang, Xiufeng Zhang, Shangguo Hou*. Single molecule spectrum dynamics imaging with 3D target-locking tracking. Nature Communications. 2025. Accepted.

2. Shiyi Qin†, Zhi Yang†, Huimin Liu, Xiaoli Wang, Shangguo Hou*, Kai Huang*. Binding memory of liquid molecules. Nature Communications. 2025. 16, 6555  https://doi.org/10.1038/s41467-025-61630-3

3. Ri Zhou#, Hao Sha#, Shengjie Fu#, Guannan Liu, Jinbei Wei, Chenguang Wang*, Shangguo Hou*, Geyu Lu*. Hyperspectral Fluorescence Imaging with A New Polarity-Ultrasensitive Fluorescent Probe. Advanced Science. 2025, e08792  https://doi.org/10.1002/advs.202508792

4. Huanlong Zheng#, Hao Sha#, Ri Zhou#, Yu Wu, Chenguang Wang*, Shangguo Hou*, Geyu Lu*. Rational development of Nile Red derivatives with significantly improved specificity and photostability for advanced fluorescence imaging of lipid droplets. Biosensors and Bioelectronics, 2025, 117494 https://doi.org/10.1016/j.bios.2025.117494

5. Haoyang Li†, Quan Lu†, Zhong Wang†, Wenbo Zhang, Yu Wu, Yandong Sun, Dongping Zhong*, Suhui Deng* and Shangguo Hou*. Three-dimensional random-access confocal microscopy with 3D remote focusing system. Communications Engineering, 3, 166 (2024) https://doi.org/10.1038/s44172-024-00320-2,  Selected in topical collection "Microscopic Imaging in Deep Tissue"

6. Zhaoshuai Gao, Qian Li, Chunhai Fan*, Shangguo Hou*. Deciphering live-cell biomolecular dynamics with single-molecule fluorescence imaging. Science Bulletin. 2024, 69(12), 1823-1828. https://doi.org/10.1016/j.scib.2024.03.036

7. Zhaoshuai Gao†, Shangguo Hou†, Suhui Deng†, Le Liang, Fei Wang, Linjie Guo, Weina Fang, Qian Li, Bin Kang*, Hong-Yuan Chen*, Chunhai Fan*. Non-linear scanning switch-off microscopy for super-resolution fluorescence imaging. Nano Letters, 2024 24 (39), 12125-12132

8. Hao Sha, Haoyang Li, Yongbing Zhang*, Shangguo Hou*. Deep learning-enhanced single-molecule spectrum imaging. APL Photonics, 2023, 8, 096102.

9. Xiufeng Zhang†, Haoyang Li†, Yue Ma†, Dongping Zhong*, and Shangguo Hou*. Study Liquid-liquid phase separation with optical microscopy: a methodology review. APL Bioengineering, 2023; 7(2): 021502. (Featured Article)

10. Shangguo Hou, Jack Exell, Kevin Welsher. Real-time 3D Single Molecule Tracking. Nature Communications, 2020, 11,3607  https://doi.org/10.1038/s41467-020-17444-6