Our research focuses on developing next-generation multi-dimensional biological imaging techniques and using them to explore the mystery of the complex biological system. Including:

1. multi-dimensional biological imaging techniques development;

2. the molecular mechanism of pathogen-host interactions;

3. Quantitative biomolecular dynamic imaging;

4. AI-assisted biological imaging.

Dr. Hou Shangguo’s research focuses on the development of high spatiotemporal resolution optical microscopy techniques and their applications in biological systems. He has developed a series of multi-dimensional imaging methods to study the interactions between biomolecules and cells under various systems and conditions, addressing molecular and cellular systems at different scales. Shangguo’s work has been published in many high impact journals including Nature Communications, Science Bulletin, Small, Optics Letters, APL Photonics and Optics Express. The research work has been positively cited by prestigious journals such as Nature Photonics, Molecular Cell, and ACS Nano,and has been highlighted multiple times by academic websites such as Phys.org and Duke Research. He has been a member of American Biophysical Society and American Association for the Advancement of Science since 2016 and 2019, respectively.

Representative papers:

Zhaoshuai Gao, Qian Li, Chunhai Fan*, Shangguo Hou*. Deciphering live-cell biomolecular dynamics with single-molecule fluorescence imaging. Science Bulletin. 2024. https://doi.org/10.1016/j.scib.2024.03.036. (Accepted).

Hao Sha, Haoyang Li, Yongbing Zhang*, Shangguo Hou*. Deep learning-enhanced single-molecule spectrum imaging. APL Photonics, 2023, 8 (9): 096102.

Xiufeng Zhang†, Haoyang Li†, Yue Ma†, Dongping Zhong*, and Shangguo Hou*. Study Liquid-liquid phase separation with optical microscopy: a methodology review. APL Bioengineering, 2023, 7(2): 021502. 

Shangguo Hou, Jack Exell, Kevin Welsher*. Real-time 3D Single Molecule Tracking. Nature Communications, 2020, 11, 3607

Shangguo Hou, Kevin Welsher*. An Adaptive Real-Time 3D Single Particle Tracking Method for Monitoring Viral First Contacts. Small, 2019, 15, 1903039

Shangguo Hou, Xiaoqi Lang, Kevin Welsher*. Robust real-time 3D single-particle tracking using a dynamically moving laser spot. Optics Letters, 2017, 42, 2390-2393.

Xiaochen Tan, Shangguo Hou, Anastasia Niver, Chen Zhang, Alexis Johnson, and Kevin D. Welsher* Active-feedback 3D single-molecule tracking using a fast-responding galvo scanning mirror. J. Phys. Chem. A., 2023. 217, 30, 6320-6328

Zhichao Liu, Ying Zhu, Liming Zhang, Weiping Jiang, Yawei Liu, Qiaowei Tang, Xiaoqing Cai, Jiang Li, Lihua Wang, Changlu Tao, Xianzhen Yin, Xiaowei Li, Shangguo Hou, Dawei Jiang, Kai Liu, Xin Zhou*, Hongjie Zhang*, Maili Liu*, Chunhai Fan*, Yang Tian*. Structural and functional imaging of brains. SCIENCE CHINA Chemistry. 2023, 66, 324–366

Siddharth Patel, N Ashwanikumar, Ema Robinson, Yan Xia, Cosmin Mihai, Joseph Griffith III, Shangguo Hou, Adam Esposito, Tatiana Ketova, Kevin Welsher, John Joyal, Orn Almarsson, Gaurav Sahay*. Naturally-occurring cholesterol analogues in lipid nanoparticles induce polymorphic shape and enhance intracellular delivery of mRNA. Nature Communications, 2020, 11, 983