范潇课题组将立足于多学科交叉领域，发挥技术优势，开发新型冷冻电镜成像技术及高效样品制备与数据处理方法，构建针对天然膜体系的膜蛋白原位结构与功能解析平台。并将依托该平台开展复杂生物膜环境的高效原位研究，通过多维度解析关键大分子的空间构型、相互作用网络及跨尺度组织模式，深入探索生物膜系统间的耦合与调控机制，为相关疾病干预提供理论基础。

范潇博士长期致力于融合多学科前沿技术方法，以推动冷冻电镜技术创新，并聚焦于重大疾病相关膜蛋白-药物分子相互作用机制的结构生物学研究。通过自主研发的冷冻电镜全流程创新技术体系，克服了该领域长期存在的技术瓶颈，成功实现了超小分子量蛋白及近生理状态下膜蛋白的高分辨率结构解析，并揭示了电压门控钠离子/钙离子通道的结构与药理作用机制等一系列重要发现。

人类前沿科学计划（HFSP）博士后基金奖

* Equal contributions # Corresponding author

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[2] Fan, X.*, Huang, J.*#, Yang, L.*, Chen, J., Wang, H., Huang, X., Geng, J., Wu, Q., Xie, Y., Lu, F., Guo, Q., Shen, Z., Jin, X.# and Yan, N.#. Diverse binding poses of agonistic neurotoxins on human Nav1. 6. Nature 2026, pp.1-10. https://doi.org/10.1038/s41586-026-10661-x

[3] Fan, X.*#, Chen, J.*, Xue, L.*, Wang, H., Wu, T., Huang, X., Lu, F., Jin, X., Song, C., Huang, J.# and Yan, N.#. Open-state structure of veratridine-activated human Nav1. 7 reveals the molecular choreography of fast inactivation. Vita 2026, 1(1): 19-31. https://doi.org/10.15302/vita.2026.01.0003

[4] Fan, X.*, Chen, J*., Huang, X., Hou, Z., Xie, Y., Li, Z., Yan, N.# and Huang, J.#. Phrixotoxin-3 binds to three distinct antagonistic sites on human Nav1. 6. Cell Research 2025, 35(8): 610-613.

[5] Yang, Z.*, Fan, X.* and Wang, H.W.#. Near-atomic resolution single-particle 3D reconstruction using a single Cryo-EM micrograph. Journal of Chinese Electron Microscopy Society 2025, 44 (1): 1000-6281.

[6] Huang, J.*, Fan, X.*#, Jin, X., Lyu, C., Guo, Q., Liu, T., Chen, J., Davakan, A., Lory, P. and Yan, N.#. Structural basis for human Cav3. 2 inhibition by selective antagonists. Cell Research 2024, 34(6): 440-450.

[7] Yang, Z., Fan, J., Wang, J., Fan, X., Ouyang, Z., Wang, H.W.# and Zhou, X.#. Electrospray-assisted cryo-EM sample preparation to mitigate interfacial effects. Nature Methods 2024, 21(6): 1023-1032.

[8] Fan, X. *#, Huang, J.*, Jin, X. and Yan, N.#. Cryo-EM structure of human voltage-gated sodium channel Nav1. 6. Proceedings of the National Academy of Sciences 2023, 120(5): p.e2220578120.

[9] Huang, J.*, Fan, X.*#, Jin, X., Teng, L. and Yan, N.#, 2023. Dual-pocket inhibition of Nav channels by the antiepileptic drug lamotrigine. Proceedings of the National Academy of Sciences 2023, 120(41): p.e2309773120.

[10] Wu, Q.*, Huang, J.*#, Fan, X.*#, Wang, K.*, Jin, X., Huang, G., Li, J., Pan, X.# and Yan, N.#. Structural mapping of Nav1. 7 antagonists. Nature communications 2023, 14(1): 3224.

[11] Huang, J.*, Fan, X.*, Jin, X., Jo, S., Zhang, H.B., Fujita, A., Bean, B.P.# and Yan, N.#. Cannabidiol inhibits Nav channels through two distinct binding sites. Nature communications 2023, 14(1): 3613.

[12] Shen, J.*, Hu, M.*, Fan, X.*#, Ren, Z., Portioli, C., Yan, X., Rong, M. and Zhou, M.#. Extracellular domain of PepT1 interacts with TM1 to facilitate substrate transport. Structure 2022, 30(7): 1035-1041.

[13] Ni, H.*, Fan, X.*, Zhou, F., Guo, G., Lee, J.Y., Seeman, N.C., Kim, D.N., Yao, N., Chaikin, P.M. and Han, Y.#. Direct visualization of floppy two-dimensional DNA origami using cryogenic electron microscopy. iScience 2022, 6(25): 104373.

[14] Yao, X.*, Fan, X.*# and Yan, N.#. Cryo-EM analysis of a membrane protein embedded in the liposome. Proceedings of the National Academy of Sciences 2020, 117(31): 18497-18503.

[15] Han, Y.*, Fan, X.*, Wang, H., Zhao, F., Tully, C.G., Kong, J., Yao, N. and Yan, N.#. High-yield monolayer graphene grids for near-atomic resolution cryoelectron microscopy. Proceedings of the National Academy of Sciences 2020, 117(2): 1009-1014.

[16] Fan, X.*, Wang, J.*, Zhang, X., Yang, Z., Zhang, J.C., Zhao, L., Peng, H.L., Lei, J. and Wang, H.W., 2019. Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution. Nature Communications 2019, 10(1): 2386.

[17] Wang, H.W. and Fan, X. Challenges and opportunities in cryo-EM with phase plate. Current Opinion in Structural Biology 2019, 58: 175-182.

[18] Fan, X.*, Zhao, L.*, Liu, C., Zhang, J.C., Fan, K., Yan, X., Peng, H.L., Lei, J.# and Wang, H.W.#. Near-atomic resolution structure determination in over-focus with volta phase plate by Cs-corrected cryo-EM. Structure 2017, 25(10):1623-1630.

课题组正在招聘副研究员/助理研究员、博士后。