侯尚国课题组聚焦于高时空分辨光学显微成像技术的开发及其在复杂生命过程中的应用，致力于建立的新一代智能单分子显微成像平台。课题组建立了实时三维单分子主动追踪、多维荧光光谱与寿命成像、目标锁定体积成像、内容自适应与事件触发成像等多种成像技术，以实现对运动生物分子的三维轨迹、扩散状态、局部微环境和分子相互作用的长时间、低光损、定量化同步测量，并将这些方法应用于生物分子凝聚体、病毒—受体相互作用、线粒体自噬与分裂、脂滴代谢、膜结构重塑及疾病相关蛋白动态等重要生命科学问题。

我们以成像技术创新推动重要生物学问题的解决，同时以生物学需求牵引成像方法的进一步发展，形成技术与科学问题相互促进的良性循环。组员在科研过程中获得了搭建乐高积木和专业摄影般的快乐，并满足探究生命活动运行奥秘的好奇心。

研究方向：

1. 多维度、多尺度先进光学显微成像技术开发；

2. 生物分子凝聚体中单分子动力学及调控机制的解析；

3. 人工智能驱动的智能显微成像与自适应测量技术；

病原体与宿主互作过程定量及机制解析。

2022：广东省珠江人才计划-青年拔尖人才

2021：深圳市高层次人才

2020：杜克大学Fitzpatrick光子学研究所杰出博后讲座奖

2019：杜克大学杰出博后提名奖

2015：上海市优秀毕业生

1. Hao Sha#, Yu Wu#, Yongbing Zhang#*, Ran Liu, Xiaochen Feng, Haoyang Li, Zhong Wang, Xiufeng Zhang, Shangguo Hou*. Single molecule spectrum dynamics imaging with 3D target-locking tracking. Nature Communications. 2025, 16, 8686 (2025). https://doi.org/10.1038/s41467-025-63787-3  新闻报道

2. Shiyi Qin#, Zhi Yang#, Huimin Liu, Xiaoli Wang, Shangguo Hou*, Kai Huang*. Binding memory of liquid molecules. Nature Communications. 2025, 16, 6555. https://doi.org/10.1038/s41467-025-61630-3

3. Ri Zhou#, Hao Sha#, Shengjie Fu#, Guannan Liu, Jinbei Wei, Chenguang Wang*, Shangguo Hou*, Geyu Lu*. Hyperspectral Fluorescence Imaging with A New Polarity-Ultrasensitive Fluorescent Probe. Advanced Science. 2025, 12, e08792, https://doi.org/10.1002/advs.202508792

4. Huanlong Zheng#, Hao Sha#, Ri Zhou#, Yu Wu, Chenguang Wang*, Shangguo Hou*, Geyu Lu*. Rational development of Nile Red derivatives with significantly improved specificity and photostability for advanced fluorescence imaging of lipid droplets. Biosensors and Bioelectronics, 2025, 282, 117494

5. Zhaoshuai Gao, Qian Li, Chunhai Fan*, Shangguo Hou*. Deciphering live-cell biomolecular dynamics with single-molecule fluorescence imaging. Science Bulletin. 2024, 69(12), 1823-1828. https://doi.org/10.1016/j.scib.2024.03.036

6. Chen Zhang#*, Ran Liu#, Zichen Ding, Peng Lu, Weiming Tian, Yan Zhao, Jiaye He, Shangguo Hou*, Review of Machine Learning for Single-Particle Tracking: Methods, Challenges, and Biophysical Insights. Chemical & Biomedical Imaging. 4(5), 695–718 (2026). (selected as ACS Editors' Choice !) https://doi.org/10.1021/cbmi.5c00146

7. Haoyang Li†, Quan Lu†, Zhong Wang†, Wenbo Zhang, Yu Wu, Yandong Sun, Dongping Zhong*, Suhui Deng* and Shangguo Hou*. Three-dimensional random-access confocal microscopy with 3D remote focusing system. Communications Engineering, 3, 166 (2024) https://doi.org/10.1038/s44172-024-00320-2,  Selected in topical collection "Microscopic Imaging in Deep Tissue"

8. Hao Sha, Haoyang Li, Yongbing Zhang*, Shangguo Hou*. Deep learning-enhanced single-molecule spectrum imaging. APL Photonics, 2023, 8 (9): 096102.

9. Shangguo Hou†*, Chen Zhang†, Anastasia Niver, Kevin D Welsher*. Mapping nanoscale forces and potentials in live cells with microsecond 3D single-particle tracking. Chemical & Biomedical Imaging, 2026,  https://pubs.acs.org/doi/full/10.1021/cbmi.5c00293

10. Shangguo Hou, Jack Exell, Kevin Welsher. Real-time 3D Single Molecule Tracking. Nature Communications, 2020, 11, 3607 (Reported by Duke Research) 

11. Xiufeng Zhang#, Jingqi Zhang#, Luyao Yan, Ran Liu, Junhui Huang, Shangguo Hou*, Xuefeng Guo*, Dongping Zhong*. Elucidation of Structural Fluctuation for Initial Signaling and DNA Recognition for Damage Repair of a Bifunctional Cryptochrome. The Journal of Physical Chemistry Letters, 2026,17(14),4181-4189. https://doi.org/10.1021/acs.jpclett.6c00602

12.  Xing Jiang#, Xiaoli Wang#, Song Shen, Shangguo Hou*, Chen Yu*, 3D Genome Engineering: Current Advances and Therapeutic Opportunities in Human Diseases. Research, 2025, 8, 0865 DOI:10.34133/research.0865

13. Quan Lu, Yu Wu, Mei Ting Mei, Yue Hu*, Shangguo Hou*, Advances in Three-Dimensional Single-Molecule Tracking Microscopy with Closed-loop Feedback Control (Invited). Laser & Optoelectronics Progress, 2025, 62(18), 1817005. Invited paper

14. Zhaoshuai Gao†, Shangguo Hou†, Suhui Deng†, Le Liang, Fei Wang, Linjie Guo, Weina Fang, Qian Li, Bin Kang*, Hong-Yuan Chen*, Chunhai Fan*. Non-linear scanning switch-off microscopy for super-resolution fluorescence imaging. Nano Letters, 2024 24 (39), 12125-12132

15. Xiufeng Zhang†, Haoyang Li†, Yue Ma†, Dongping Zhong*, and Shangguo Hou*. Study Liquid-liquid phase separation with optical microscopy: a methodology review. APL Bioengineering, 2023, 7(2): 021502.  (Featured Article!)